Siffofin Lactoperoxidase

Lactoperoxidase (LPO), wanda aka samo shi a cikin gland na salivary da mammary gland, abu ne mai mahimmanci na martanin rigakafi mai mahimmanci a cikin kula da lafiyar baka. Babban mahimmancin lactoperoxidase shine don ion oxideze ionioyanyan ion (SCN−) wanda aka samo a cikin ƙira a gaban hydrogen peroxide wanda ke haifar da samfuran da ke nuna ayyukan antimicrobial. LPO da aka samo a cikin madarar bovine an yi amfani dashi a cikin masana'antar kiwon lafiya, abinci, da masana'antu na kwaskwarima saboda aikinsa da kuma tsarin kamanceceniyar mutum.

Ana wadatar da samfuran tsabta na yau da kullun tare da tsarin lactoperoxidase don ba da kyakkyawar madadin zuwa daidaitaccen ƙwayar haƙoran foloide. Saboda manyan aikace-aikace na ƙarin lactoperoxidase, buƙatarta ta karu sosai a cikin shekaru, kuma har yanzu tana ci gaba.
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Menene Lactoperoxidase?

Lactoperoxidase shine peroxidase enzyme da aka samar daga gabobin mucosal, dabbobi masu shayarwa, da kuma ciwan hanji, wanda yake aiki azaman wakilin maganin kwayan cuta na halitta. A cikin mutane, an sanya lazamin lactoperoxidase ta hanyar abubuwan LPO. Wannan enzyme yawanci ana samun shi a cikin dabbobi masu shayarwa, wadanda suka hada da mutane, mice, bovine, raƙumi, saniya, saniya, akuya, Ilama, da tumaki.

Aikin Lactoperoxidase:

LPO wakili ne mai inganci na gaske. Abubuwan Lactoperoxidase sune, saboda haka, dangane da wannan ka'idodin. Aikace-aikacen Lactoperoxidase ana samo shi ta musamman a tanadin abinci, hanyoyin maganin ophthalmic, da dalilai na kwaskwarima. Hakanan, an yi amfani da foda lactoperoxidase a cikin rauni da magani na hakori. Bugu da ƙari, LPO ingantacciyar ƙwayar cuta ce mai hana ƙwayoyin cuta da ƙwayar cuta. Tattaunawa da ke ƙasa akwai ƙarin amfani da lactoperoxidase:

i. Ciwon daji na nono

Lactoperoxidase cancer ikon gudanarwa yana da alaƙa da ikon sa na oxidize estradiol. Wannan hadawan abu da iskar shaka yana haifar da damuwa da iskar shaka a cikin kwayoyin cutar kansa. Aikin Lactoperoxidase anan shine haifar da jerin abubuwanda zasu haifar da yawan iskar oxygen da kuma tarin hydrogen peroxide. A sakamakon waɗannan halayen, LPO yadda yakamata ya kashe ƙwayoyin tumor a cikin fitiro. Hakanan, macrophages da aka nuna wa LPO suna aiki don rusa ƙwayoyin kansa, suna kashe su.

ii. Tasirin anti-kwayan cuta

Hasken enzyme na LPO yana aiki a matsayin mahallin halitta na tsarin garkuwar jiki na rigakafi na dabbobi masu shayarwa kuma yana kama da iskar shaka daga cikin abubuwan da ke faruwa a cikin kwayoyin cututtukan cututtukan kwayoyin. LPO na iya hana haɓakar ƙwayoyin ƙwayoyin cuta ta hanyar tasirin enzymatic wanda ya shafi ionioyanyan ion da hydrogen peroxide a matsayin cofactors. Ayyukan antimicrobial na LPO an tsara shi akan haɓakar ions hypothiocyanite ta hanyar kunna enzymes. Hypothiocyanite ion sun sami damar amsawa tare da ƙwayoyin ƙwayoyin cuta. Suna kuma haifar da rudani a cikin aiki na tsoffin enzymes na rayuwa. Lactoperoxidase yana kashe kwayoyin cuta na Gram-korau kuma yana hana ci gaba da haɓaka ƙwayoyin cuta masu ƙwayar cuta ta Gram.

iii. Lactoperoxidase a cikin kayan kwaskwarima

Haɗin lactoperoxidase foda, glucose, thiocyanate, iodide,

da kuma glucose oxidase, kuma an san su da tasiri a cikin adon kayan kwalliya.Lactoperoxidas-02

iv. Lactoperoxidase a cikin madara adana

Establishedarfin lactoperoxidase a cikin tsabtataccen ingancin madara mai tsabta na ɗan lokaci an kafa shi a cikin fannoni da dama da kuma nazarin gwaji da aka gudanar a yankuna daban-daban na ƙasa. Za'a iya amfani da ƙwayoyin Lactoperoxidase don adana ƙwayar madara da aka samo daga jinsuna daban. Yadda tasiri hanya ya dogara da dalilai da yawa. Wadannan abubuwan sun hada da yawan zafin jiki na madara a lokacin jiyya, nau'in gurbataccen kwayoyin cuta, da kuma yawan madara.

Lactoperoxidase yana haifar da tasirin ƙwayar cuta a cikin madara mai ɗumama. Bayanai na bincike da gogewa daga aiki na zahiri sun nuna cewa ana iya amfani da lactoperoxidase sama da iyakar zazzabi na (15-30 digiri Celsius) wanda aka ambata a cikin jagororin kundin adireshi na 1991. A mafi ƙarancin ƙarshen ma'aunin zafin jiki, bincike daban-daban ya nuna cewa kunna lactoperoxidase na iya jinkirtar da ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta ta madara kuma don haka jinkirta ɓarke ​​da madara don ƙarin kwanaki idan aka kwatanta da firiji kaɗai. Yana da mahimmanci a lura cewa dalilin amfani da lactoperoxidase ba shine ya sanya madara ta zama mai wadatar amfani ba amma don adana ingancin sa.

Yin aiki da tsabta mai kyau a cikin samar da madara yana da mahimmanci ga ingancin lactoperoxidase kuma ga ingancin madara mai ƙwayoyi. Aminci da ɗanɗano madara za'a iya samu kawai ta haɗuwa da zafi na madara da kyawawan halayen tsabta masu zaman kansu ba tare da amfani da lactoperoxidase ba.

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v. Wasu ayyuka

Nazarin ya nuna cewa ban da samun tasirin rigakafi, Lactoperoxidase kuma yana iya kare sel dabbobi daga lalacewar iri da kuma peroxidation, sannan kuma muhimmin bangare ne na tsarin tsaro na kariya daga cututtukan cututtukan kwayoyin cuta a cikin tsarin narkewar jarirai.

Tsarin Lactoperoxidase

Menene Tsarin Lactoperoxidase?

Tsarin Lactoperoxidase (LPS) ya ƙunshi abubuwa uku, waɗanda suka haɗa da lactoperoxidase, hydrogen peroxide, da thiocyanate (SCN¯). Tsarin Lactoperoxidase yana da ayyukan hana ƙwayoyin cuta lokacin da waɗannan abubuwan ukun suka yi aiki tare. A cikin amfani da rai na ainihi, idan maida hankali kan wani sashi a cikin tsarin bai isa ba, dole ne a kara shi don tabbatar da tasirin ƙwayar cuta, wanda aka sani da kunnawa LPS. Daga cikin su, lactoperoxidase maida hankali kada ya kasance kasa da 0.02 U / mL.

Hankalin lactoperoxidase na halitta a cikin madara mai bovine shine 1.4 U / mL, wanda zai iya biyan wannan buƙata. SCN¯ ana samunsa sosai a cikin ɓoye da kyallen dabbobi. A cikin madara, yawan thiocyanate yana ƙima kamar 3-5 μg / mL. Wannan lamari ne mai iyakancewa ga aikin tsarin Lactoperoxidase. An ba da shawarar cewa thiocyanate da ake buƙata don kunna tsarin lactoperoxidase ya kusan 15 μg / mL ko ma fiye da haka. Abin da ya sa muke buƙatar ƙara wannan thiocyanate exogenous don kunna tsarin lactoperoxidase. Abun hydrogen peroxide a cikin madara, wanda aka fitar dashi, shine 1-2 μg / Ml ne kawai, kuma kunna LPS yana buƙatar 8-10 XNUMX-XNUMXg / mL na hydrogen peroxide. Abin da ya sa ya kamata a samar da hydrogen peroxide a waje.

Tsarin lactoperoxidase yana taka muhimmiyar rawa a cikin tsarin rigakafin halittar jiki, yana iya kashe kwayoyin cuta a cikin madara da kuma mucosal secretion kuma yana iya samun aikace-aikacen warkewa.

A cikin kayayyakin abinci da na kiwon lafiya, ana iya amfani da ƙari ko haɓaka tsarin lactoperoxidase don sarrafa ƙwayoyin cuta.

Yaya ta yi aiki?

LPS ta ƙunshi ƙirƙirar ƙwayar rigakafin ƙwayar cuta daga SCN¯catalysed ta LPO a gaban hydrogen peroxide. Ya ce lactoperoxidase antimicrobial aiki ana samunsa ta dabi'a a cikin ruwa mai yawa kamar ruwan 'ya'yan ciki, hawaye, da yau. Abubuwa biyu masu mahimmanci don tsarin maganin rigakafi, waɗanda sune hydrogen peroxide da thiocyanate, suna cikin madara a cikin nau'ikan abubuwa daban-daban, dangane da nau'in dabba da abincin da aka bayar.

A cikin madara mai sabo, aikin antimicrobial yana da rauni kuma ya kasance na ɗan awanni 2 tunda madara kawai ta ƙunshi matakan suboptimal na hydrogen peroxide da ion thiocyanate ion. An ƙara Thiocyanate wanda aka oxidized a cikin 2 electron dauki dake samar da hypothiocyanite

Ayyukan Thiocyanate azaman cofactor na tsarin Lactoperoxidase. Sakamakon haka, adadin abubuwan oxidized sulfhydryls a duka yana da 'yanci daga ion thiocyanate muddin dai

  1. Akwai Thiol moiety
  2. Thiocyanate bai gaji ba
  • Isasshen hydrogen peroxide yana nan
  1. Har yanzu ba'a haɗa Thiocyanate cikin amino acid na ƙanshi ba

A sakamakon haka, thiocyanate yana sake inganta tasirin ƙwayar cuta na tsarin lactoperoxidase a cikin madara mai sabo. Wannan yana shimfiɗa rayuwar shiryayye na madara mai sabo a ƙarƙashin yanayin wurare na wurare bakwai zuwa takwas.

Aikace-aikacen Lactoperoxidase / Yana amfani

i. Anti-microbial Action

Ayyukan anti-microbial na tsarin lactoperoxidase ana gani a cikin ƙwayoyin cuta da aiki na kwayan cuta na wasu ƙananan ƙwayoyin cuta da aka samo a cikin madara mai narkewa. Tsarin ƙwayoyin cuta nasa yana aiki a cikin wannan rukunin thiol da aka samo akan ƙwayoyin ƙwayar ƙwayar ƙwayar plasma sel yana oxidized. Wannan yana haifar da rushewar ƙwayar ƙwayar plasma wanda ke haifar da fashewar polypeptides, ion potassium, da amino acid. Rage abubuwa na purines da pyrimidines, glucose, da amino acid ta sel an hana su. Amfani da kwayar halitta na DNA, RNA, da sunadarai suma ana hana su.

Kwayoyin cuta daban-daban suna nuna matakan digiri daban-daban na tsarin lactoperoxidase. Kwayoyin cuta na ƙwayoyin cuta, kamar su Salmonella, Pseudomonas, da Escherichia coli, an hana su kuma ana kashe su. Kwayoyin Lactic acid da Streptococcus an hana su. Halakar waɗannan ƙwayoyin cuta ta tsarin lactoperoxidase yana haifar da yaduwar wasu abubuwan gina jiki, yana hana ƙwayoyin cuta amfani da abubuwan gina jiki, kuma wannan yana haifar da raguwa ko mutuwar ƙwayoyin cuta.

ii. Jiyya na paradentosis, gingivitis, da kashe ƙwayoyin tumor

LPS An yi imanin zai yi tasiri a cikin jiyya na gingivitis da paradentosis. Anyi amfani da LPO a cikin matse bakinsa don rage ƙwayoyin bakin mutum kuma, a sakamakon haka, acid ɗin da waɗannan ƙwayoyin cuta suka samar. Antibody conjugates na lactoperoxidase da glucose oxidase sun nuna suna da tasiri wajen ruguzawa saboda haka suna kashe ƙwayoyin tumor a cikin fitsari. Hakanan, macrophages da aka fallasa ga tsarin lactoperoxidase suna aiki don lalata da kashe ƙwayoyin daji.

iii. Kulawar Magani

Nazarin asibiti daban-daban da ke bayyana amfanin LPS a cikin haƙoran haƙora. Bayan nuna kai tsaye, ta yin amfani da ma'auni na yanayin yanayin gwajin, cewa lactoperoxidase likitan haƙoran da ke ɗauke da amyloglucosidase (γ-amylase) yana da fa'ida cikin fa'idar kulawa. Enzymes kamar su glucose oxidase, lysozyme, da lactoperoxidase ana canjawa wuri kai tsaye daga likin hakori zuwa cikin murfin kumbura.

Kasancewartattun sassan magana, wadannan enzymes suna da karfi sosai a aikace. Hakanan, LPS yana da amfani mai amfani don hanawa game da ƙwararrun ƙananan yara ta hanyar rage yawan dauloli waɗanda aka kirkiro ta microflora cariogenic yayin da suke haɓaka taro na thiocyanate.

Tare da marasa lafiyar xerostomia, lactoperoxidase hakori ya fi kyau idan aka kwatanta da fluoride na hakori idan ya zo ga kirkirar plasta. Aikace-aikacen LPS ba a taƙaita shi ga cututtukan cututtukan cututtukan fata ba. Za'a iya amfani da haɗin lactoperoxidase da lysozyme a cikin maganin ƙonewar bakin ciki na ciwo.

Lokacin da aka haɗa LPS tare da lactoferrin, wannan haɗin yana haɗuwa da halitosis. Lokacin da aka haɗa LPS tare da lysozyme da lactoferrin, LPS tana taimaka wajan inganta alamun bayyanar cututtukan xerostomia. Hakanan, mala'ikan da ke cikin tsarin lactoperoxidase suna taimakawa haɓaka bayyanar cututtukan cututtukan daji yayin da aka hana yin amfani da ƙwayar cuta ta hanzari.

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iv. Inganta tsarin garkuwar jiki

Lactoperoxidase antimicrobial aiki yana taka muhimmiyar rawa a cikin tsarin rigakafi. Hypothiocyanite shine mai amsawa wanda aka samar da ayyukan lactoperoxidase akan thiocyanate. Hydrogen peroxide yana samar da sunadarai na Duox2 (dual oxidase 2). Thiocyanate secretion a cikin marasa lafiya da cystic fibrosis an saukar da su. Wannan yana haifar da raguwa a cikin samar da hypothiocyanite na antimicrobial. Wannan yana taimakawa ga haɗarin cutar kamuwa da iska.

LPS tana hana helicobacter pylori aiki sosai. Amma a cikin kullun ɗan adam, LPS yana nuna raunin anti-ƙwayar cuta mai rauni. LPS ba ta kaiwa hari ga DNA kuma ba mutagenic bane. Amma, a cikin takamaiman yanayi, LPS na iya haifar da ɗan damuwa damuwa na rashin ƙarfi. An tabbatar da cewa LPO a cikin kasancewar thiocyanate na iya haifar da tasirin cytotoxic da kwayoyin cuta na hydrogen peroxide a cikin yanayi musamman, gami da lokacin da H2O2 ya kasance a cikin gaurayawar amsawar a cikin yawan ƙwayoyin thiocyanate.

Bugu da kari, saboda da karfi da kuma ingancin antibacterial kaddarorin da babban zafi juriya, ana amfani da shi azaman antibacterial wakili don rage al'ummomin kwayar cuta a cikin madara ko kayayyakin madara da kuma a matsayin mai nuna madara ultra-pasteurization. Ta kunna tsarin lactoperoxidase, za a iya tsawaita rayuwar rayuwar madara mai sanyaya abinci mai tsafta.

Kuma, hypothiocyanate da aka samar ta lactoperoxidase za a iya amfani dashi don hana kwayar cutar herpes simplex da kwayar cuta ta mutum.

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Shin lafiya ga lafiyar mutum da dabba?

Shekaru goma sha biyar na nazarin filayen a cikin kasashe masu tasowa da ci gaba an gudanar da kuma binciken da Hukumar FAO / WHO JECFA (Kwamitin Kwararru kan Abincin Abinci). Bayan an kammala wannan binciken mai zurfi da kuma mahimman bayanai, hukumar FAO / WHO JECFA (Kwamitin Kwararru game da Abincin Abinci) ya amince da amfani da tsarin lactoperoxidase wajen adana madara. Masana sun kuma bayyana wannan hanyar a zaman lafiya ga lafiyar dan adam da na dabbobi.

LPS yanki ne na dabi'un ruwan zazzabin ciki da na yau da kullun a cikin mutane kuma, saboda haka, amintaccen lokacin da aka yi amfani da shi a bin ka'idodi na Hukumar Codex Alimentarius. Wannan hanyar ba ta tasiri dabbobi masu larurar komai. Wannan saboda ana yin magani ne kawai bayan an fitar da madara daga ɗakin.

Kammalawa

Ya tabbata daga tattaunawarmu cewa tsarin lactoperoxidase da lactoperoxidase suna da fa'ida kuma suna da fa'ida sosai a aikace da yawa. Idan kana neman cikawa saya lactoperoxidase don bincikenku ko haɓaka magunguna, kada ku duba gaba. Muna da ikon aiwatar da babban umarni na lactoperoxidase a cikin mafi kankanin lokaci zai yiwu kuma mu jigilar su zuwa Amurka, Turai, Kanada, da sauran wasu sassan duniya. Barka da zuwa tuntuɓar mu don ƙarin bayani.

References

  1. Jantschko, PG Furtmüller, M. Allegra et al., "Redox tsaka-tsakin tsirrai da tsirrai na mammalian: binciken kwatancen na wucin gadi-kayyakin aikinsu ne game da abubuwanda suka haifar," Archives of Biochemistry da Biophysics, vol. 398, ba. 1, shafi 12-22, 2002.
  2. Tenovuo JO (1985). Tsarin peroxidase a cikin sirrin mutane. " A cikin Tenovuo JO, Pruitt KM (eds.). Tsarin Lactoperoxidase: ilmin sunadarai da mahimmancin halitta. New York: Dekker. p 272.
  3. Thomas EL, Bozeman PM, Koyi DB: Lactoperoxidase: tsari da kaddarorin kayan masarufi. Peroxidases a cikin Chemistry da Biology. An gyara shi: Everse J, Everse KE, Grisham MB. 1991, Boca Raton, FL. Latsa CRC, 123-142.
  4. Wijkstrom-Frei C, El-Chemaly S, Ali-Rachedi R, Gerson C, Cobas MA, Forteza R, Salathe M, Conner GE (Agusta 2003). “Lactoperoxidase da kare mutuncin hanyar hajiyar dan adam”. Am. J. Respir. Mol Cell Biol. 29 (2): 206-12.
  5. Mikola H, Waris M, Tenovuo J: Haramcin nau'in ƙwayar cuta ta nau'in ƙwayar cuta ta 1, ƙwayar ƙwayar ƙwayar ƙwayar mahaifa, da nau'in echovirus 11 ta hypothiocyanite-peroxidase. Magungunan rigakafi 1995, 26 (2): 161-171.
  6. Haukioja A, Ihalin R, Loimaranta V, Lenander M, Tenovuo J (Satumba 2004). "Sirrin Helicobacter pylori zuwa tsarin kariya na rayuwa, tsarin lactoperoxidase, a cikin buffer da kuma dukkan jijiyar mutum". Jaridar Nazarin Kiwon Lafiya. 53 (Pt 9): 855-60.

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