Siffofin Lactoperoxidase

Lactoperoxidase (LPO), wanda aka samo shi a cikin gland na salivary da mammary gland, abu ne mai mahimmanci na martanin rigakafi mai mahimmanci a cikin kula da lafiyar baka. Babban mahimmancin lactoperoxidase shine don ion oxideze ionioyanyan ion (SCN−) wanda aka samo a cikin ƙira a gaban hydrogen peroxide wanda ke haifar da samfuran da ke nuna ayyukan antimicrobial. LPO da aka samo a cikin madarar bovine an yi amfani dashi a cikin masana'antar kiwon lafiya, abinci, da masana'antu na kwaskwarima saboda aikinsa da kuma tsarin kamanceceniyar mutum.

Ana wadatar da samfuran tsabta na yau da kullun tare da tsarin lactoperoxidase don ba da kyakkyawar madadin zuwa daidaitaccen ƙwayar haƙoran foloide. Saboda manyan aikace-aikace na ƙarin lactoperoxidase, buƙatarta ta karu sosai a cikin shekaru, kuma har yanzu tana ci gaba.
Lactoperoxidas-01

Menene Lactoperoxidase?

Lactoperoxidase shine peroxidase enzyme da aka samar daga gabobin mucosal, dabbobi masu shayarwa, da kuma ciwan hanji, wanda yake aiki azaman wakilin maganin kwayan cuta na halitta. A cikin mutane, an sanya lazamin lactoperoxidase ta hanyar abubuwan LPO. Wannan enzyme yawanci ana samun shi a cikin dabbobi masu shayarwa, wadanda suka hada da mutane, mice, bovine, raƙumi, saniya, saniya, akuya, Ilama, da tumaki.

 

Aikin Lactoperoxidase:

LPO wakili ne mai inganci na gaske. Abubuwan Lactoperoxidase sune, saboda haka, dangane da wannan ka'idodin. Aikace-aikacen Lactoperoxidase ana samo shi ta musamman a tanadin abinci, hanyoyin maganin ophthalmic, da dalilai na kwaskwarima. Hakanan, an yi amfani da foda lactoperoxidase a cikin rauni da magani na hakori. Bugu da ƙari, LPO ingantacciyar ƙwayar cuta ce mai hana ƙwayoyin cuta da ƙwayar cuta. Tattaunawa da ke ƙasa akwai ƙarin amfani da lactoperoxidase:

 

i. Ciwon daji na nono

Lactoperoxidase ciwon daji ikon gudanarwa yana da alaƙa da ikon sa na oxidize estradiol. Wannan hadawan abu da iskar shaka yana haifar da damuwa da iskar shaka a cikin kwayoyin cutar kansa. Aikin Lactoperoxidase anan shine haifar da jerin abubuwanda zasu haifar da yawan iskar oxygen da kuma tarin hydrogen peroxide. A sakamakon waɗannan halayen, LPO yadda yakamata ya kashe ƙwayoyin tumor a cikin fitiro. Hakanan, macrophages da aka nuna wa LPO suna aiki don rusa ƙwayoyin kansa, suna kashe su.

 

ii. Tasirin anti-kwayan cuta

L enzyme na LPO yana aiki ne a matsayin mahaɗan halitta na tsarin kare halittu masu kariya na dabbobi masu shayarwa kuma yana haifar da iskar shaka na ion thiocyanate cikin cikin antibacterial hypothiocyanate. LPO na iya hana haɓakar ƙananan ƙwayoyin cuta ta hanyar maganin enzymatic wanda ya haɗa da ion thiocyanate da hydrogen peroxide a matsayin cofactors. Ayyukan antimicrobial na LPO suna da ka'ida akan samuwar ions hypothiocyanite ta hanyar kunna enzymes. Ion Hypothiocyanite suna iya amsawa tare da membranes na ƙwayoyin cuta. Hakanan suna haifar da rushewa a cikin aiki na musamman enzymes na rayuwa. Lactoperoxidase yana kashe ƙwayoyin cuta na Gram-negative kuma yana hana haɓaka da ci gaban ƙwayoyin Gram-tabbatacce.

 

iii. Lactoperoxidase a cikin kayan kwaskwarima

Haɗin lactoperoxidase foda, glucose, thiocyanate, iodide,

da kuma glucose oxidase, kuma an san su da tasiri a cikin adon kayan kwalliya.Lactoperoxidas-02

 

 

 

 

 

 

iv. Lactoperoxidase a cikin madara adana

Establishedarfin lactoperoxidase a cikin tsabtataccen ingancin madara mai tsabta na ɗan lokaci an kafa shi a cikin fannoni da dama da kuma nazarin gwaji da aka gudanar a yankuna daban-daban na ƙasa. Za'a iya amfani da ƙwayoyin Lactoperoxidase don adana ƙwayar madara da aka samo daga jinsuna daban. Yadda tasiri hanya ya dogara da dalilai da yawa. Wadannan abubuwan sun hada da yawan zafin jiki na madara a lokacin jiyya, nau'in gurbataccen kwayoyin cuta, da kuma yawan madara.

Lactoperoxidase yana aiki da tasirin kwayar cuta a cikin ɗanyen madara mai ɗanɗano. Bayanan bincike da gogewa daga aikin gaske sun nuna cewa ana iya amfani da lactoperoxidase bayan iyakancin zafin jiki na (15-30 digiri Celsius) wanda aka ambata a cikin jagororin codex na 1991. A mafi ƙarancin ƙarshen sikelin sikelin, mabanbanta karatu ya nuna cewa kunna lactoperoxidase na iya jinkirta haɓakar ƙwayar madara ta psychrotrophic don haka jinkirta ɓarnatar da madara don ƙarin kwanaki idan aka kwatanta da firinji kadai. Yana da mahimmanci a lura cewa manufar yin amfani da lactoperoxidase ba wai don sanya madara mai lafiya don amfani bane amma don adana asalin sa.

Yin aiki da tsabta mai kyau a cikin samar da madara yana da mahimmanci ga ingancin lactoperoxidase kuma ga ingancin madara mai ƙwayoyi. Aminci da ɗanɗano madara za'a iya samu kawai ta haɗuwa da zafi na madara da kyawawan halayen tsabta masu zaman kansu ba tare da amfani da lactoperoxidase ba.

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v. Wasu ayyuka

Nazarin ya nuna cewa ban da samun tasirin rigakafi, Lactoperoxidase kuma yana iya kare sel dabbobi daga lalacewar iri da kuma peroxidation, sannan kuma muhimmin bangare ne na tsarin tsaro na kariya daga cututtukan cututtukan kwayoyin cuta a cikin tsarin narkewar jarirai.

 

Tsarin Lactoperoxidase

Menene Tsarin Lactoperoxidase?

Tsarin Lactoperoxidase (LPS) ya ƙunshi abubuwa uku, waɗanda suka haɗa da lactoperoxidase, hydrogen peroxide, da thiocyanate (SCN¯). Tsarin Lactoperoxidase yana da ayyukan hana ƙwayoyin cuta lokacin da waɗannan abubuwan ukun suka yi aiki tare. A cikin amfani da rai na ainihi, idan maida hankali kan wani sashi a cikin tsarin bai isa ba, dole ne a kara shi don tabbatar da tasirin ƙwayar cuta, wanda aka sani da kunnawa LPS. Daga cikin su, lactoperoxidase maida hankali kada ya kasance kasa da 0.02 U / mL.

Tsarin lactoperoxidase na halitta a cikin madarar bovine shine 1.4 U / mL, wanda zai iya biyan wannan buƙatar. SCN¯ yana wadatuwa a cikin ɓoyayyen abu da kayan jikin dabbobi. A cikin madara, yawan kwayar cutar ta thiocyanate kadan ne kamar 3-5 μg / mL. Wannan shine iyakantaccen yanki don aikin tsarin Lactoperoxidase. An ba da shawara cewa thiocyanate da ake buƙata don kunna tsarin lactoperoxidase yana kusa da 15 μg / mL ko ma fiye da haka. Wannan shine dalilin da ya sa muke buƙatar ƙara wannan yanayin don kunna tsarin lactoperoxidase. Abincin hydrogen peroxide a cikin madara, wanda aka fitar, 1-2 onlyg / Ml ne kawai, kuma kunna LPS yana buƙatar 8-10 μg / mL na hydrogen peroxide. Wannan shine dalilin da ya sa ya kamata a samar da hydrogen peroxide a waje.

Tsarin lactoperoxidase yana taka muhimmiyar rawa a cikin tsarin rigakafin halittar jiki, yana iya kashe kwayoyin cuta a cikin madara da kuma mucosal secretion kuma yana iya samun aikace-aikacen warkewa.

A cikin kayayyakin abinci da na kiwon lafiya, ana iya amfani da ƙari ko haɓaka tsarin lactoperoxidase don sarrafa ƙwayoyin cuta.

 

Yaya ta yi aiki?

LPS ta ƙunshi ƙirƙirar ƙwayar rigakafin ƙwayar cuta daga SCN¯catalysed ta LPO a gaban hydrogen peroxide. Ya ce lactoperoxidase antimicrobial aiki ana samunsa ta dabi'a a cikin ruwa mai yawa kamar ruwan 'ya'yan ciki, hawaye, da yau. Abubuwa biyu masu mahimmanci don tsarin maganin rigakafi, waɗanda sune hydrogen peroxide da thiocyanate, suna cikin madara a cikin nau'ikan abubuwa daban-daban, dangane da nau'in dabba da abincin da aka bayar.

A cikin madara mai sabo, aikin antimicrobial yana da rauni kuma ya kasance na ɗan awanni 2 tunda madara kawai ta ƙunshi matakan suboptimal na hydrogen peroxide da ion thiocyanate ion. An ƙara Thiocyanate wanda aka oxidized a cikin 2 electron dauki dake samar da hypothiocyanite

Ayyukan Thiocyanate azaman cofactor na tsarin Lactoperoxidase. Sakamakon haka, adadin abubuwan oxidized sulfhydryls a duka yana da 'yanci daga ion thiocyanate muddin dai

  1. Akwai wadatar Thiol
  2. Thiocyanate bai gaji ba
  • Isasshen hydrogen peroxide yana nan
  1. Har yanzu ba'a haɗa Thiocyanate cikin amino acid na ƙanshi ba

A sakamakon haka, thiocyanate yana sake inganta tasirin ƙwayar cuta na tsarin lactoperoxidase a cikin madara mai sabo. Wannan yana shimfiɗa rayuwar shiryayye na madara mai sabo a ƙarƙashin yanayin wurare na wurare bakwai zuwa takwas.

 

Aikace-aikacen Lactoperoxidase / Yana amfani

 

i. Anti-da ake dasu Aiki

Ayyukan anti-microbial na tsarin lactoperoxidase ana ganin su a cikin aikin kwayar cuta da kwayar halittar wasu ƙananan ƙwayoyin cuta waɗanda aka samo a cikin ɗanyen madara. Tsarin kwayar cuta yana aiki a cikin cewa ƙungiyar thiol da aka samo akan membrane plasma membrane ɗakuna. Wannan yana haifar da lalata tsarin membrane wanda ke haifar da zubewar polypeptides, potassium ions, da amino acid. Karɓar purines da pyrimidines, glucose, da amino acid da ƙwayoyi ke hanawa. An kuma hana kira na DNA, RNA, da sunadarai.

Kwayoyin cuta daban-daban suna nuna digiri daban-daban na ƙwarewa ga tsarin lactoperoxidase. Kwayar gram-negative, kamar Salmonella, Pseudomonas, da Escherichia coli, an hana su kuma an kashe su. Ana hana kwayoyin Lactic acid da Streptococcus kawai. Lalacewar wadannan kwayoyin cuta ta hanyar lactoperoxidase yana haifar da zubewar wasu sinadarai, yana hana kwayoyin cutar cin abinci, kuma wannan yana haifar da raguwa ko mutuwar kwayoyin cuta.

 

ii. Jiyya na paradentosis, gingivitis, da kashe ƙwayoyin tumor

LPS An yi imanin zai yi tasiri a cikin jiyya na gingivitis da paradentosis. Anyi amfani da LPO a cikin matse bakinsa don rage ƙwayoyin bakin mutum kuma, a sakamakon haka, acid ɗin da waɗannan ƙwayoyin cuta suka samar. Antibody conjugates na lactoperoxidase da glucose oxidase sun nuna suna da tasiri wajen ruguzawa saboda haka suna kashe ƙwayoyin tumor a cikin fitsari. Hakanan, macrophages da aka fallasa ga tsarin lactoperoxidase suna aiki don lalata da kashe ƙwayoyin daji.

 

iii. Kulawar Magani

Nazarin asibiti daban-daban da ke bayyana amfanin LPS a cikin haƙoran haƙora. Bayan nuna kai tsaye, ta yin amfani da ma'auni na yanayin yanayin gwajin, cewa lactoperoxidase likitan haƙoran da ke ɗauke da amyloglucosidase (γ-amylase) yana da fa'ida cikin fa'idar kulawa. Enzymes kamar su glucose oxidase, lysozyme, da lactoperoxidase ana canjawa wuri kai tsaye daga likin hakori zuwa cikin murfin kumbura.

Kasancewartattun sassan magana, wadannan enzymes suna da karfi sosai a aikace. Hakanan, LPS yana da amfani mai amfani don hanawa game da ƙwararrun ƙananan yara ta hanyar rage yawan dauloli waɗanda aka kirkiro ta microflora cariogenic yayin da suke haɓaka taro na thiocyanate.

Tare da marasa lafiyar xerostomia, lactoperoxidase hakori ya fi kyau idan aka kwatanta da fluoride na hakori idan ya zo ga kirkirar plasta. Aikace-aikacen LPS ba a taƙaita shi ga cututtukan cututtukan cututtukan fata ba. Za'a iya amfani da haɗin lactoperoxidase da lysozyme a cikin maganin ƙonewar bakin ciki na ciwo.

Lokacin da aka haɗa LPS tare da lactoferrin, wannan haɗin yana haɗuwa da halitosis. Lokacin da aka haɗa LPS tare da lysozyme da lactoferrin, LPS tana taimaka wajan inganta alamun bayyanar cututtukan xerostomia. Hakanan, mala'ikan da ke cikin tsarin lactoperoxidase suna taimakawa haɓaka bayyanar cututtukan cututtukan daji yayin da aka hana yin amfani da ƙwayar cuta ta hanzari.

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iv. Inganta tsarin garkuwar jiki

Lactoperoxidase antimicrobial aiki yana taka muhimmiyar rawa a cikin tsarin garkuwar jiki. Hypothiocyanite abu ne mai amsawa wanda aikin lactoperoxidase ya samar akan thiocyanate. Hydrogen peroxide ana samar dashi ne daga sunadaran Duox2 (dual oxidase 2). Ioarjin Thiocyanate a cikin marasa lafiya tare da cystic fibrosis an saukar da su. Wannan yana haifar da raguwa a cikin samar da maganin rigakafi na antimicrobial hypothiocyanite. Wannan yana taimakawa ga haɗarin kamuwa da iska ta iska.

LPS tana hana helicobacter pylori yadda yakamata. Amma a cikin yawan bakin ɗan adam, LPS yana nuna raunin anti-ƙwayoyin cuta. LPS ba sa kaiwa DNA hari kuma ba mutagenic bane. Amma, a ƙarƙashin yanayi na musamman, LPS na iya haifar da ɗan gajiyar ƙarancin abu. An tabbatar da cewa LPO a cikin yanayin thiocyanate na iya haifar da tasirin cytotoxic da bactericidal na hydrogen peroxide a ƙarƙashin wasu yanayi, gami da lokacin da H2O2 ya kasance a cikin cakuda abubuwan da ke faruwa fiye da thiocyanate.

Bugu da kari, saboda da karfi da kuma ingancin antibacterial kaddarorin da babban zafi juriya, ana amfani da shi azaman antibacterial wakili don rage al'ummomin kwayar cuta a cikin madara ko kayayyakin madara da kuma a matsayin mai nuna madara ultra-pasteurization. Ta kunna tsarin lactoperoxidase, za a iya tsawaita rayuwar rayuwar madara mai sanyaya abinci mai tsafta.

Kuma, hypothiocyanate da aka samar ta lactoperoxidase za a iya amfani dashi don hana kwayar cutar herpes simplex da kwayar cuta ta mutum.

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Shin lafiya ga lafiyar mutum da dabba?

Shekaru goma sha biyar na nazarin filayen a cikin kasashe masu tasowa da ci gaba an gudanar da kuma binciken da Hukumar FAO / WHO JECFA (Kwamitin Kwararru kan Abincin Abinci). Bayan an kammala wannan binciken mai zurfi da kuma mahimman bayanai, hukumar FAO / WHO JECFA (Kwamitin Kwararru game da Abincin Abinci) ya amince da amfani da tsarin lactoperoxidase wajen adana madara. Masana sun kuma bayyana wannan hanyar a zaman lafiya ga lafiyar dan adam da na dabbobi.

LPS yanki ne na halitta na ruwan ciki da na yau a cikin mutane kuma, sabili da haka, amintacce idan aka yi amfani dashi wajen bin ƙa'idodin Hukumar Codex Alimentarius. Wannan hanyar ba ta shafi dabbobi masu shayarwa kwata-kwata. Wannan saboda ana yin maganin ne bayan an fitar da madara daga nono.

 

Kammalawa

Ya tabbata daga tattaunawarmu cewa tsarin lactoperoxidase da lactoperoxidase suna da fa'ida kuma suna da fa'ida sosai a aikace da yawa. Idan kana neman cikawa saya lactoperoxidase don bincikenku ko haɓaka magunguna, kada ku duba gaba. Muna da ikon aiwatar da babban umarni na lactoperoxidase a cikin mafi kankanin lokaci zai yiwu kuma mu jigilar su zuwa Amurka, Turai, Kanada, da sauran wasu sassan duniya. Barka da zuwa tuntuɓar mu don ƙarin bayani.

 

References

  1. Jantschko, PG Furtmüller, M. Allegra et al., "Redox tsaka-tsakin tsire-tsire da na peroxidases na dabbobi: nazarin kwatankwacin halin-kwatankwacin abin da suke yi game da abin da ya shafi indole," Archives of Biochemistry and Biophysics, vol. 398, a'a. 1, shafi na 12-22, 2002.
  2. Tenovuo JO (1985). "Tsarin peroxidase a cikin sirrin dan adam." A cikin Tenovuo JO, Pruitt KM (eds.). Tsarin Lactoperoxidase: ilmin sunadarai da mahimmancin ilmin halitta. New York: Dekker. shafi na. 272.
  3. Thomas EL, Bozeman PM, Koyi DB: Lactoperoxidase: tsari da kaddarorin kayan masarufi. Peroxidases a cikin Chemistry da Biology. An gyara shi: Everse J, Everse KE, Grisham MB. 1991, Boca Raton, FL. Latsa CRC, 123-142.
  4. Wijkstrom-Frei C, El-Chemaly S, Ali-Rachedi R, Gerson C, Cobas MA, Forteza R, Salathe M, Conner GE (Agusta 2003). "Lactoperoxidase da jirgin sama na iska sun dauki bakuncin tsaro". Am. J. Respir. Cell Mol. Biol. 29 (2): 206–12.
  5. Mikola H, Waris M, Tenovuo J: Haramcin nau'in ƙwayar cuta ta nau'in ƙwayar cuta ta 1, ƙwayar ƙwayar ƙwayar ƙwayar mahaifa, da nau'in echovirus 11 ta hypothiocyanite-peroxidase. Magungunan rigakafi 1995, 26 (2): 161-171.
  6. Haukioja A, Ihalin R, Loimaranta V, Lenander M, Tenovuo J (Satumba 2004). "Sensitivity of Helicobacter pylori to a innata tsaro inji, da lactoperoxidase tsarin, a cikin buffer da kuma a cikin dukan mutum yau". Jaridar Medical Microbiology. 53 (Pt 9): 855-60.

 

 

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